Identification of small molecules affecting p53-MDM2/MDMX interaction by fluorescence polarization.
نویسندگان
چکیده
Fluorescence polarization (FP) has become a powerful technique to quantitatively analyze the binding of a small soluble fluorescence-labeled probe to a larger soluble protein and its displacement by other molecules. Here, we describe a detailed protocol to identify small molecules capable of targeting p53-MDM2/MDMX interactions using a fluorescence polarization assay with Rhodamine-labeled p53 peptides.
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ورودعنوان ژورنال:
- Methods in molecular biology
دوره 962 شماره
صفحات -
تاریخ انتشار 2013